Figure 4.
Figure 4. A cell-intrinsic T cell deficiency and competitive failure of lymphoid reconstitution. (A and B) Lethally irradiated Rag1 mutant mice (CD45.2+) were reconstituted with unmixed scanT or wild-type bone marrow (CD45.2+; A) or a mixture of scanT or wild-type marrow (CD45.2+) with wild-type bone marrow (CD45.1+; B). Chimerism was measured 8 wk after transplant. Panels in B have been gated on the indicated cell subset. (C) Lethally irradiated wild-type recipients (CD45.1+) were transplanted with an equal mixture of wild-type (CD45.1+) and heterozygous or homozygous mutant (CD45.2+) bone marrow. Wild-type donor chimerism (CD45.1+) was measured 8 wk later in the spleen, bone marrow, and thymus. Subsets were gated as in Fig. 3, with the addition of NK progenitors (NKP; 7-AAD−CD19−TCR-β−CD122+NK1.1−). (A–C) Data are representative of one (A) or three (B and C) independent experiments with at least three mice per group. Error bars represent standard error.

A cell-intrinsic T cell deficiency and competitive failure of lymphoid reconstitution. (A and B) Lethally irradiated Rag1 mutant mice (CD45.2+) were reconstituted with unmixed scanT or wild-type bone marrow (CD45.2+; A) or a mixture of scanT or wild-type marrow (CD45.2+) with wild-type bone marrow (CD45.1+; B). Chimerism was measured 8 wk after transplant. Panels in B have been gated on the indicated cell subset. (C) Lethally irradiated wild-type recipients (CD45.1+) were transplanted with an equal mixture of wild-type (CD45.1+) and heterozygous or homozygous mutant (CD45.2+) bone marrow. Wild-type donor chimerism (CD45.1+) was measured 8 wk later in the spleen, bone marrow, and thymus. Subsets were gated as in Fig. 3, with the addition of NK progenitors (NKP; 7-AAD−CD19−TCR-β−CD122+NK1.1−). (A–C) Data are representative of one (A) or three (B and C) independent experiments with at least three mice per group. Error bars represent standard error.

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