Figure 6. p53 deficiency did not rescue long-term B cell development or lymphoid progenitor defects. Lethally irradiated recipient mice (CD45.1+) were transplanted with Lin− BM cells (CD45.1−) and were treated with Tam to delete Bcl11a. Recipients were subsequently analyzed 4 wk later (n = 4/genotype). (A) Total cell numbers of donor BM B cells of indicated genotypes. (B) Total numbers of donor LSKs, LMPPs, and CLPs. (C) Freshly sorted CLPs (5,000) from mice of indicated genotypes 3 d after Tam treatment were cultured on OP9 stromal cells in the presence of 10 ng/ml of IL-7 and Flt3L for 14 d. A–C represent at least three independent experiments. *, P < 0.05; **, P < 0.01.
Figure 6.

p53 deficiency did not rescue long-term B cell development or lymphoid progenitor defects. Lethally irradiated recipient mice (CD45.1+) were transplanted with Lin− BM cells (CD45.1−) and were treated with Tam to delete Bcl11a. Recipients were subsequently analyzed 4 wk later (n = 4/genotype). (A) Total cell numbers of donor BM B cells of indicated genotypes. (B) Total numbers of donor LSKs, LMPPs, and CLPs. (C) Freshly sorted CLPs (5,000) from mice of indicated genotypes 3 d after Tam treatment were cultured on OP9 stromal cells in the presence of 10 ng/ml of IL-7 and Flt3L for 14 d. A–C represent at least three independent experiments. *, P < 0.05; **, P < 0.01.

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