Profound defects of lymphoid development in Bcl11a-deficient adult mouse. B and T cells at different developmental stages were analyzed in the BM or thymus 1 wk after the mice were injected with Tam (n = 4/genotype, and the experiment was repeated 4 times). (A) BM B cells. Numbers refer to percentages of B cells in total BM-nucleated cells. (B) Cellularity of BM B cells in mice of the indicated genotypes. Cells were collected from two femurs of each mouse. (C) Thymocytes of various development stages. Lin⁻ thymocytes were further analyzed by expression of c-Kit and CD25 for DN thymocytes. (D) Cellularity of DN thymocytes in mice of indicated genotypes. (E) Analysis of LSKs, LMPPs, CLPs, and Ly6d⁺CLPs. Numbers refer to percentages of progenitors in total BM-nucleated cells. (F) Total cell numbers. +/+, CreERT2; Bcl11a^+/+. +/flox, CreERT2; Bcl11a^+/flox. flox/flox, CreERT2; Bcl11a^flox/flox. *, P < 0.05; **, P < 0.01.