Dynamic expression patterns of Bcl11a in hematopoiesis. (A) Schematic diagram of the Bcl11a-eGFP reporter allele. The eGFP reporter cassette flanked by two F3 sites is introduced to the 3′UTR region of Bcl11a, 8 bp after the stop codon TAG. (B) Flow cytometry tracking Bcl11a expression using the Bcl11a^eGFP/eGFP reporter mice. Cell surface markers for defining these cells are described in Table S1. (C) Expression of Bcl11a (eGFP⁺) and Bcl11b (Tdtomato⁺) in double negative (DN) thymocytes was measured by flow cytometry. DN thymocytes are identified as described in Fig. S1 D. (D) qRT-PCR analysis of Bcl11a expression in sorted hematopoietic populations. Data represent mean values of three independent biological replicates and all values are normalized to the expression of the Gapdh gene. Error bars indicate the SD. Thy, thymus; PB, peripheral blood; M, macrophages; G, granulocytes; Mk, megakaryocytes; B, BM CD19⁺ B cells; T, spleen CD3⁺T cells. In all flow cytometry assays, at least four mice were analyzed for each cell type in independent experiments.