Figure 4. Constitutive activation of RBP-J signaling in myeloid osteoclast precursor compartment in mice suppresses inflammatory bone resorption. (A) μCT analysis of the femurs of 8-wk-old male control or NICD1M mice was performed. BV/TV, trabecular bone volume/tissue volume; Tb.N, trabecular number; Tb.Th, trabecular bone thickness; Tb.Sp, trabecular separation. n = 5 in each group. NS, no statistically significant difference. (B) TRAP staining of histological sections and (C) the concentration of serum TRAP obtained from 6-wk-old NICD1M and control mice with or without supracalvarial TNF administration (100 µg/kg body weight). Bar, 50 µm. n = 5 per group. **, P < 0.01. (D) Histomorphometric analysis of calvaria from 6-wk-old NICD1M and control mice. N.Oc/BS, number of osteoclasts per bone surface; Oc.S/BS, osteoclast surface per bone surface. n = 5 per group. **, P < 0.01. (E) Paw swelling in arthritis induced in the 6-wk-old control and NICDM mice using anticollagen antibodies as described in Materials and methods. For each mouse, paw swelling was calculated as the sum of measurements of joint thickness of two wrists and two ankles. Paw swelling is represented as the mean ± SD for each group. n = 5 per group. NS, no statistically significant difference. (F) H&E staining of tissue sections of tarsal joints in 6-wk-old control and NICD1M mice treated with PBS or arthritis-inducing monoclonal antibody cocktail (arthritis) as described in Materials and methods. Black arrowheads indicate bone erosions. Bar, 100 µm. (G) TRAP staining of the tissue sections from the same area labeled with a dotted box in F. Black arrows show the osteoclasts (red spots) in the resorptive area. Bar, 50 µm. (H) Serum TRAP levels obtained from control and NICD1M mice at indicated times before and after induction of arthritis. n = 5 per group. **, P < 0.01. (I and J) Quantitative histomorphometric analysis of bone erosion (I) and osteoclast numbers (J) in tarsal joints. n = 5 per group. **, P < 0.01. Data are representative of two independent experiments (A–J).
Figure 4.

Constitutive activation of RBP-J signaling in myeloid osteoclast precursor compartment in mice suppresses inflammatory bone resorption. (A) μCT analysis of the femurs of 8-wk-old male control or NICD1M mice was performed. BV/TV, trabecular bone volume/tissue volume; Tb.N, trabecular number; Tb.Th, trabecular bone thickness; Tb.Sp, trabecular separation. n = 5 in each group. NS, no statistically significant difference. (B) TRAP staining of histological sections and (C) the concentration of serum TRAP obtained from 6-wk-old NICD1M and control mice with or without supracalvarial TNF administration (100 µg/kg body weight). Bar, 50 µm. n = 5 per group. **, P < 0.01. (D) Histomorphometric analysis of calvaria from 6-wk-old NICD1M and control mice. N.Oc/BS, number of osteoclasts per bone surface; Oc.S/BS, osteoclast surface per bone surface. n = 5 per group. **, P < 0.01. (E) Paw swelling in arthritis induced in the 6-wk-old control and NICDM mice using anticollagen antibodies as described in Materials and methods. For each mouse, paw swelling was calculated as the sum of measurements of joint thickness of two wrists and two ankles. Paw swelling is represented as the mean ± SD for each group. n = 5 per group. NS, no statistically significant difference. (F) H&E staining of tissue sections of tarsal joints in 6-wk-old control and NICD1M mice treated with PBS or arthritis-inducing monoclonal antibody cocktail (arthritis) as described in Materials and methods. Black arrowheads indicate bone erosions. Bar, 100 µm. (G) TRAP staining of the tissue sections from the same area labeled with a dotted box in F. Black arrows show the osteoclasts (red spots) in the resorptive area. Bar, 50 µm. (H) Serum TRAP levels obtained from control and NICD1M mice at indicated times before and after induction of arthritis. n = 5 per group. **, P < 0.01. (I and J) Quantitative histomorphometric analysis of bone erosion (I) and osteoclast numbers (J) in tarsal joints. n = 5 per group. **, P < 0.01. Data are representative of two independent experiments (A–J).

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