Role of TRPC6 in mediating LPS-induced mortality and proinflammatory cytokine production in mice. (A) Mice were challenged i.p. (40 mg/kg body weight) with LPS and their survival was assessed by log-rank test using 15 mice in each group. (B) Mice were sacrificed ∼25 h after i.p. injection of LPS (40 mg/kg body weight), and the levels of proinflammatory cytokines were determined in lung lysates using mouse Bio-Plex Multiplex Cytokine Assay kit (Bio-Rad Laboratories). Data represent mean ± SEM from three independent experiments. * indicates values significantly higher than values in WT or TRPC6^−/− lungs without LPS (P < 0.05). ** indicates values significantly lower than values in WT lungs after LPS (P < 0.05). (C) CLP was induced in WT and TRPC6^−/− mice as described in Materials and methods, and their survival was assessed using log-rank test. A total of 15 mice were used in each group. (D) CLP-induced cytokine generation in lungs was determined after 22 h of CLP. Data represent mean ± SEM from three independent experiments. * indicates values significantly higher than values in WT lungs without CLP. ** indicates values significantly lower than values in WT lungs after CLP (P < 0.05).