Intracellular localization of murine ALR proteins. (A) 500 ng ALR-HA expression plasmids alone (left two columns) or 500 ng ALR-HA, 500 ng ASC-eGFP, and 500 ng STING-FLAG (right columns) were transfected into 3.0 × 10⁴ HeLa cells and probed 24 h after transfection with anti-HA-ALR (red), anti-eGFP (green), anti-FLAG (blue), and DAPI (gray). (B) HA-tagged murine MNDA was immunoprecipitated from control cells and cells expressing STING-FLAG and ASC-FLAG, which can be distinguished based on their size despite the same tag. (C) HA-tagged Pyr-A was immunoprecipitated from cells expressing the indicated constructs, followed by blotting for STING-FLAG and ASC-FLAG. All data are representative of three to five independent experiments.