Figure 7. Failure of genetic ablation of p53 in BMKO mice to restore serum Ig levels, Ig class-switching, and B1a cells. (A) Serum IgM (left) and IgG1 (right) levels in unimmunized mice of the indicated genotypes were determined by ELISA. Horizontal bars are the geometric mean. Results shown are from a single experiment involving 6–10 mice per genotype. (B) Purified splenic B cells from mice of the indicated genotypes were stimulated with LPS plus IL-4 for 4 d and the percentage of IgG1+ class-switched B cells was determined by flow cytometry. Numbers indicate the percentage of class-switched B cells among gated B cells. (C) The percentage of B1a cells (CD5+B220+) in the peritoneal cavity of mice of the indicated genotypes was determined by flow cytometry. For B and C, results are representative of two to three independent experiments involving one to three mice per genotype.
Figure 7.

Failure of genetic ablation of p53 in BMKO mice to restore serum Ig levels, Ig class-switching, and B1a cells. (A) Serum IgM (left) and IgG1 (right) levels in unimmunized mice of the indicated genotypes were determined by ELISA. Horizontal bars are the geometric mean. Results shown are from a single experiment involving 6–10 mice per genotype. (B) Purified splenic B cells from mice of the indicated genotypes were stimulated with LPS plus IL-4 for 4 d and the percentage of IgG1+ class-switched B cells was determined by flow cytometry. Numbers indicate the percentage of class-switched B cells among gated B cells. (C) The percentage of B1a cells (CD5+B220+) in the peritoneal cavity of mice of the indicated genotypes was determined by flow cytometry. For B and C, results are representative of two to three independent experiments involving one to three mice per genotype.

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