In vivo administration of CD40 agonist in RAG2^−/−x γc^−/− mice induces effective immunoediting and intratumoral M1 macrophages. Regressor cell line 2 was transplanted into RAG2^−/−x γc^−/− mice receiving a single dose of either control rat IgG or anti-CD40 agonistic monoclonal antibodies on day 5. Tumor growth was measured over time. (A) Tumor masses were converted into passaged daughter cell lines which were transplanted into syngeneic WT mice and assessed for tumor formation (number of cell lines and mice are indicated in the figure). Tumor-free mice were defined to have a nonenlarging mass <9 mm in average diameter by day 40. (B) At day 15 after transplantation, tumor masses were disaggregated into single-cell suspensions, and the percentage of M1 (top) and M2 (bottom) macrophages of CD11b⁺ events for each condition were quantified. *, P < 0.05; ***, P < 0.001. Error bars are represented by ± SEM. Each symbol represents a different mouse. Results were reproduced at least once.