Figure 2. Enhanced activity of TNF... | Rockefeller University Press

Figure 2.

$Figure 2. Enhanced activity of TNF receptor–deficient HSCs after transplantation. (a–f) BM cells were pooled from three Tnfrsf1-dKO (CD45.2+) or three WT (CD45.1+) mice (8–12 wk old), and for each of the genotypes 106 unfractionated BM cells were competitively cotransplanted into lethally irradiated congenic WT recipients (CD45.1+/2+). Recipient mice were evaluated for PB multilineage reconstitution levels at the indicated time points. (a) Representative FACS analysis illustrating PB myeloid chimerism evaluation. (b) PB myeloid reconstitution derived from transplanted Tnfrsf1-dKO and WT cells was analyzed at the indicated time points. At the time points indicated by the vertical dashed lines, mice were sacrificed, and 0.5 femur equivalent of BM cells were serially transplanted into newly irradiated (CD45.1+/2+) recipients. Displayed are PB myeloid chimerism levels. Results are mean (SEM) values from six separate experiments with five to seven recipients in each experiment. (c–f) 16 wk after the primary transplantation, PB analysis of T (CD4/CD8), myeloid (MAC1), and B (B220) cell reconstitution was performed (d), BM was analyzed for LSK chimerism (f), and mean percentages for all recipients are presented. (c and e) Representative FACS analysis for reconstitution levels within the PB and BM are shown. (d and f) Results are from six separate experiments with five to seven recipients in each experiment. Mean (SEM) values are shown. (g–j) BM cells from 8–12 wk old Tnfrsf1a−/− (g and i) and Tnfrsf1b−/− (h and j) mice (CD45.2+, backcrossed for 10 generations with C57BL/6 mice) were transplanted in competition with WT BM cells (CD45.1+) into lethally irradiated WT (CD45.1+/2+) recipients. PB myeloid chimerism levels (g and h) and BM LSK chimerism levels (i and j) at the indicated time points after primary and secondary (indicated by dashed line) transplantations are shown. All results are mean (SEM) values from two separate experiments with five to seven recipients in each experiment. PB and BM reconstitution analysis were performed as illustrated in panels a and e, respectively.$

Enhanced activity of TNF receptor–deficient HSCs after transplantation. (a–f) BM cells were pooled from three Tnfrsf1-dKO (CD45.2⁺) or three WT (CD45.1⁺) mice (8–12 wk old), and for each of the genotypes 10⁶ unfractionated BM cells were competitively cotransplanted into lethally irradiated congenic WT recipients (CD45.1⁺/2⁺). Recipient mice were evaluated for PB multilineage reconstitution levels at the indicated time points. (a) Representative FACS analysis illustrating PB myeloid chimerism evaluation. (b) PB myeloid reconstitution derived from transplanted Tnfrsf1-dKO and WT cells was analyzed at the indicated time points. At the time points indicated by the vertical dashed lines, mice were sacrificed, and 0.5 femur equivalent of BM cells were serially transplanted into newly irradiated (CD45.1⁺/2⁺) recipients. Displayed are PB myeloid chimerism levels. Results are mean (SEM) values from six separate experiments with five to seven recipients in each experiment. (c–f) 16 wk after the primary transplantation, PB analysis of T (CD4/CD8), myeloid (MAC1), and B (B220) cell reconstitution was performed (d), BM was analyzed for LSK chimerism (f), and mean percentages for all recipients are presented. (c and e) Representative FACS analysis for reconstitution levels within the PB and BM are shown. (d and f) Results are from six separate experiments with five to seven recipients in each experiment. Mean (SEM) values are shown. (g–j) BM cells from 8–12 wk old Tnfrsf1a^−/− (g and i) and Tnfrsf1b^−/− (h and j) mice (CD45.2⁺, backcrossed for 10 generations with C57BL/6 mice) were transplanted in competition with WT BM cells (CD45.1⁺) into lethally irradiated WT (CD45.1⁺/2⁺) recipients. PB myeloid chimerism levels (g and h) and BM LSK chimerism levels (i and j) at the indicated time points after primary and secondary (indicated by dashed line) transplantations are shown. All results are mean (SEM) values from two separate experiments with five to seven recipients in each experiment. PB and BM reconstitution analysis were performed as illustrated in panels a and e, respectively.

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