The IKKβ(EE)^IEC small intestinal LP is infiltrated with F4/80 and CD3⁺ cells. (a) Frozen sections of WT or IKKβ(EE)^IEC jejunum and colon were stained for the indicated markers and visualized by immunofluorescence. Representative of five experiments. (b) RNAs from WT or IKKβ(EE)^IEC enterocytes were subjected to gene expression profiling using whole-genome oligonucleotide arrays (Agilent). Shown are the expression levels of chemokine genes. Positive or negative values represent fold changes between IKKβ(EE)^IEC mice and littermate controls (n = 2). (c) Small intestinal IELs and LPLs were isolated from WT or IKKβ(EE)^IEC mice and counted. Percentages of F4/80 and CD3⁺ cells were determined by flow cytometry in cells gated for CD45. Results are means ± SEM (n = 6). *, P < 0.05 versus WT. Data are representative of three independent experiments.