Figure 4. IL-21 influences GC and EFPB responses from the early stages of B cell activation. (A) Representative flow cytometric dot plots showing HEL binding versus B220 staining of Il21r+/+ or Il21r−/− CD45.2 SWHEL B cells at the indicated days after transfer into congenic (CD45.1) C57BL/6 recipients immunized with HEL2x-SRBC at the time of transfer. The gates in each plot are drawn around GC B cells (B220hi; right) and EFPBs (B220lo; left). Numbers in plots indicate the percentage of HEL-binding CD45.2 SWHEL cells out of total splenocytes. (B and C) Number of SWHEL GC B cells (B) and EFPBs (C) in the same mice using the gates shown in A. These data are representative of two independent experiments with three mice per group per time point. The p-values were calculated using two-way analysis of variance that interrogates the variance over the entire time course between the two groups of mice receiving either Il21r+/+ or Il21r−/− SWHEL B cells.
Figure 4.

IL-21 influences GC and EFPB responses from the early stages of B cell activation. (A) Representative flow cytometric dot plots showing HEL binding versus B220 staining of Il21r+/+ or Il21r−/− CD45.2 SWHEL B cells at the indicated days after transfer into congenic (CD45.1) C57BL/6 recipients immunized with HEL2x-SRBC at the time of transfer. The gates in each plot are drawn around GC B cells (B220hi; right) and EFPBs (B220lo; left). Numbers in plots indicate the percentage of HEL-binding CD45.2 SWHEL cells out of total splenocytes. (B and C) Number of SWHEL GC B cells (B) and EFPBs (C) in the same mice using the gates shown in A. These data are representative of two independent experiments with three mice per group per time point. The p-values were calculated using two-way analysis of variance that interrogates the variance over the entire time course between the two groups of mice receiving either Il21r+/+ or Il21r−/− SWHEL B cells.

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