Figure 2. Reduced plasma cell responses to NP-KLH in the absence of T cell–expressed Bcl-6. (A–D) Representative flow cytometric contour plots (A and C) and quantification (B and D) of GC B cells identified as B220+ PNAhi splenocytes (A and B) and Tfh cells identified as CXCR5hi PD-1hi (gated on CD4+ cells; C and D) from 80% Bcl6−/− CD45.2:20% Cd28−/− CD45.1 (i.e., effector T cells lack Bcl-6; Tc Bcl6−/−) or control 80% Bcl6+/+ CD45.2:20% Cd28−/− CD45.1 (Tc Bcl6+/+) fetal liver chimeric mice immunized 7 d earlier with NP-KLH. (E) NP-specific IgG1 and NP-specific IgM antibodies in the same chimeric mice. (F) Photomicrographs of spleen sections stained with B220 (follicles; blue) and IgG1 (plasma cells; brown). These data are representative of two independent experiments with five mice per group in each cohort.
Figure 2.

Reduced plasma cell responses to NP-KLH in the absence of T cell–expressed Bcl-6. (A–D) Representative flow cytometric contour plots (A and C) and quantification (B and D) of GC B cells identified as B220+ PNAhi splenocytes (A and B) and Tfh cells identified as CXCR5hi PD-1hi (gated on CD4+ cells; C and D) from 80% Bcl6−/− CD45.2:20% Cd28−/− CD45.1 (i.e., effector T cells lack Bcl-6; Tc Bcl6−/−) or control 80% Bcl6+/+ CD45.2:20% Cd28−/− CD45.1 (Tc Bcl6+/+) fetal liver chimeric mice immunized 7 d earlier with NP-KLH. (E) NP-specific IgG1 and NP-specific IgM antibodies in the same chimeric mice. (F) Photomicrographs of spleen sections stained with B220 (follicles; blue) and IgG1 (plasma cells; brown). These data are representative of two independent experiments with five mice per group in each cohort.

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