Figure 1. Human Mϕs induce terminal differentiation of PCs ex vivo. (A) Close localization of CD138+ PCs and CD163+ resident tonsillar Mϕs on a human tonsil section. Red indicates CD138-AF568, and green indicates CD163-FITC. Similar images were obtained from three different tonsils from different donors. Bar, 20 µm. (B) Sorted tonsillar naive (CD27−IgD+) or memory (CD27+IgD−) B cells were labeled with CFSE and cultured alone or co-cultured with autologous Mϕs for 6 d and analyzed for CFSE dilution by flow cytometry. (C) IgA and IgG production in the supernatants of 12-d co-culture of tonsillar naive B cells and Mϕs, as measured by ELISA. Data are representative of three independent experiments using tonsils from different donors. Mean ± SD of triplicate cultures is shown.
Figure 1.

Human Mϕs induce terminal differentiation of PCs ex vivo. (A) Close localization of CD138+ PCs and CD163+ resident tonsillar Mϕs on a human tonsil section. Red indicates CD138-AF568, and green indicates CD163-FITC. Similar images were obtained from three different tonsils from different donors. Bar, 20 µm. (B) Sorted tonsillar naive (CD27−IgD+) or memory (CD27+IgD−) B cells were labeled with CFSE and cultured alone or co-cultured with autologous Mϕs for 6 d and analyzed for CFSE dilution by flow cytometry. (C) IgA and IgG production in the supernatants of 12-d co-culture of tonsillar naive B cells and Mϕs, as measured by ELISA. Data are representative of three independent experiments using tonsils from different donors. Mean ± SD of triplicate cultures is shown.

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