Figure 5. The early immune response is increased in FcγRIIbwild/H1 KI mice. (A) NP-specific IgM and IgG1 antibody serum titers were determined by ELISA 6 and 8 d after immunization with NP-KLH. (B) NP-specific IgM and IgG1 AFCs were enumerated by ELISPOT at day 8 in the spleen. Data are representative of at least two independent experiments. (C) NP-specific IgG1 AFCs were enumerated by ELISPOT at day 11 in the spleen and the BM. Two experiments have been pooled. (D) NP-specific IgM and IgG3 antibody serum titers were determined by ELISA 2, 6, and 12 d after immunization with NP-Ficoll. (E) Splenic NP-specific IgG3 and IgM AFCs were enumerated by ELISPOT 12 d after immunization. In all panels, error bars represent SEM, and p-values were determined using the Mann–Whitney two-tailed test with a risk of 5%. RU, relative units.
Figure 5.

The early immune response is increased in FcγRIIbwild/H1 KI mice. (A) NP-specific IgM and IgG1 antibody serum titers were determined by ELISA 6 and 8 d after immunization with NP-KLH. (B) NP-specific IgM and IgG1 AFCs were enumerated by ELISPOT at day 8 in the spleen. Data are representative of at least two independent experiments. (C) NP-specific IgG1 AFCs were enumerated by ELISPOT at day 11 in the spleen and the BM. Two experiments have been pooled. (D) NP-specific IgM and IgG3 antibody serum titers were determined by ELISA 2, 6, and 12 d after immunization with NP-Ficoll. (E) Splenic NP-specific IgG3 and IgM AFCs were enumerated by ELISPOT 12 d after immunization. In all panels, error bars represent SEM, and p-values were determined using the Mann–Whitney two-tailed test with a risk of 5%. RU, relative units.

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