Figure 2. Egr2 deletion results in reduced DGK-α up-regulation upon anergy induction. (A and B) OVA-specific CAR Tg × Egr2flox/flox Th1 T cells were transduced with an EV or a Cre-expressing adenovirus (Cre) to delete Egr2. The cells were then untreated or anergized with immobilized anti-CD3 for 3–6 h, and deletion of Egr2 was confirmed by qRT-PCR (A) and immunoblotting (B). (C and D) Upon Egr2 deletion, the association of Egr2 with DGK-α was determined by ChIP Assay using primers specific for DGK-α and control primers for GJA5 (C). The up-regulation of DGK-α was assessed by qRT-PCR (D). Data are presented as mean ± SD and are representative of two to three independent experiments. **, P < 0.01.
Figure 2.

Egr2 deletion results in reduced DGK-α up-regulation upon anergy induction. (A and B) OVA-specific CAR Tg × Egr2flox/flox Th1 T cells were transduced with an EV or a Cre-expressing adenovirus (Cre) to delete Egr2. The cells were then untreated or anergized with immobilized anti-CD3 for 3–6 h, and deletion of Egr2 was confirmed by qRT-PCR (A) and immunoblotting (B). (C and D) Upon Egr2 deletion, the association of Egr2 with DGK-α was determined by ChIP Assay using primers specific for DGK-α and control primers for GJA5 (C). The up-regulation of DGK-α was assessed by qRT-PCR (D). Data are presented as mean ± SD and are representative of two to three independent experiments. **, P < 0.01.

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