Analysis of dominant tolerance in vaccinated NOD mice. CFSE dilution profiles of insulin B:9-23 TCR transgenic T cells at day 4 after transfer into nonvaccinated or vaccinated 8- (a) or 30-wk-old (b) NOD mice. (c) Percentages of divided insulin B:9-23 TCR transgenic T cells after transfer into the vaccination groups as described in a and b. Bars represent means ± SEM from two independent experiments. n = 4 per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus insulin B:9-23. (d) Analysis of proliferation of T cells from pancreatic lymph nodes of NOD Foxp3-GFP reporter mice at 20 wk, vaccinated with either insulin B:9-23 or insulin mimetope 3 at 4–6 wk of age. Proliferation before or after depletion of Foxp3⁺ T cells in response to the insulin mimetope was analyzed. Results are the means ± SEM from two independent experiments. n = 4 per group.