Figure 6. Microcluster fusion and dynamics depend on ERM remodeling. (A–C) DT40 B cells expressing Ezrin-WT (A), Ezrin-CA (B), or Ezrin-DN (C) were settled onto planar lipid bilayers containing antigen (Ag) and visualized by TIRFM. GFP and antigen images are pseudocolored according to the lookup tables on the right. Images in the right panels are magnified time sequence images of the left panel (boxed areas). White ovals indicate antigen microcluster and corresponding location with respect to ezrin (black ovals). (D–F) Antigen microclusters were tracked in cells expressing Ezrin-WT, Ezrin-CA, and Ezrin-DN. (D) Representative examples of microcluster tracks during cell contraction. (E and F) Diffusion coefficients with the median indicated under the x axis and by the red bars (E) and cumulative probability plots of antigen microclusters (F). ***, P < 0.0001. Data are representative of at least two independent experiments. Bars, 2 µm.
Figure 6.

Microcluster fusion and dynamics depend on ERM remodeling. (A–C) DT40 B cells expressing Ezrin-WT (A), Ezrin-CA (B), or Ezrin-DN (C) were settled onto planar lipid bilayers containing antigen (Ag) and visualized by TIRFM. GFP and antigen images are pseudocolored according to the lookup tables on the right. Images in the right panels are magnified time sequence images of the left panel (boxed areas). White ovals indicate antigen microcluster and corresponding location with respect to ezrin (black ovals). (D–F) Antigen microclusters were tracked in cells expressing Ezrin-WT, Ezrin-CA, and Ezrin-DN. (D) Representative examples of microcluster tracks during cell contraction. (E and F) Diffusion coefficients with the median indicated under the x axis and by the red bars (E) and cumulative probability plots of antigen microclusters (F). ***, P < 0.0001. Data are representative of at least two independent experiments. Bars, 2 µm.

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