Figure 2.
Figure 2. Tetramer staining proves a specific trimolecular interaction among CD1b, GMM, and the clonotypic TCR. (a) The LDN5 T cell clone was stained with unloaded CD1b tetramers or CD1b tetramers loaded with C32 GMM. Tetramers were preincubated with 10 µg/ml isotype control antibody or anti-CD1b antibody. (b) Soluble TCR-α chains with hexahistidine tags and -β chains with streptavidin tags were formed into soluble TCR dimers. (c) Loaded and unloaded tetramers were preincubated with fivefold molar excess of soluble recombinant T cell receptors derived from CD1a-restricted (sCD8-2) or CD1b-restricted (sLDN5) T cell lines. Data are representative of three or more experiments.

Tetramer staining proves a specific trimolecular interaction among CD1b, GMM, and the clonotypic TCR. (a) The LDN5 T cell clone was stained with unloaded CD1b tetramers or CD1b tetramers loaded with C32 GMM. Tetramers were preincubated with 10 µg/ml isotype control antibody or anti-CD1b antibody. (b) Soluble TCR-α chains with hexahistidine tags and -β chains with streptavidin tags were formed into soluble TCR dimers. (c) Loaded and unloaded tetramers were preincubated with fivefold molar excess of soluble recombinant T cell receptors derived from CD1a-restricted (sCD8-2) or CD1b-restricted (sLDN5) T cell lines. Data are representative of three or more experiments.

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