Figure 1. Low avidity K b /Ova-specific... | Rockefeller University Press

Figure 1.

$Figure 1. Low avidity Kb/Ova-specific T cells in the periphery of Rip-mOva mice. Rip-mOva or transgene negative littermate control C57BL/6 mice were infected with Lm-Ova. A fraction of the mice were rechallenged 4 wk later with VSV-Ova. 6 d after the last infection, splenocytes were isolated and either left untreated or were briefly in vitro restimulated with SIINFEKL peptide. In A, CD8+ gated flow cytometry plots of cells intracellularly stained for IFN-γ, and in B, the frequencies of Ova-specific T cells are depicted (n = 3–4 mice per group). Dose–response curves graphing peptide concentration against the fraction of maximum IFN-γ response in splenic CD8+ T cells are shown in C (n = 4 and 5 per group, error bars show SD). The data shown in A–C are representative of three independently performed experiments. (D) Calculated mean half-maximum effective concentrations (EC50) obtained in three independent experiments (each including three to five mice per group) are presented. (E) Maximum blood glucose levels between days 7 and 13 after the secondary infection. The data are pooled from eight independent experiments (total number of mice: 56 Rip-mOva and 26 littermate control, transgene-negative C57BL/6 mice). The horizontal bars in B and D represent the mean value of the individual data points.$

Low avidity K^b/Ova-specific T cells in the periphery of Rip-mOva mice. Rip-mOva or transgene negative littermate control C57BL/6 mice were infected with Lm-Ova. A fraction of the mice were rechallenged 4 wk later with VSV-Ova. 6 d after the last infection, splenocytes were isolated and either left untreated or were briefly in vitro restimulated with SIINFEKL peptide. In A, CD8⁺ gated flow cytometry plots of cells intracellularly stained for IFN-γ, and in B, the frequencies of Ova-specific T cells are depicted (n = 3–4 mice per group). Dose–response curves graphing peptide concentration against the fraction of maximum IFN-γ response in splenic CD8⁺ T cells are shown in C (n = 4 and 5 per group, error bars show SD). The data shown in A–C are representative of three independently performed experiments. (D) Calculated mean half-maximum effective concentrations (EC₅₀) obtained in three independent experiments (each including three to five mice per group) are presented. (E) Maximum blood glucose levels between days 7 and 13 after the secondary infection. The data are pooled from eight independent experiments (total number of mice: 56 Rip-mOva and 26 littermate control, transgene-negative C57BL/6 mice). The horizontal bars in B and D represent the mean value of the individual data points.