Reduced colony formation ability and impaired engrafting capacity of Gimap5-deficient hematopoietic progenitors. (A) BM cells from wild-type and Gimap5-deficient mice were plated in duplicate or triplicate with the indicated cytokines, and colonies were scored at day 4 (for Epo + IL3), day 6 (for IL3 + IL6 + SCF), and day 12 (for the rest). Data shown are representative of three distinct experiments. *, P < 0.01. (B) The indicated cells were sorted from BM of wild-type mice. Total RNA was isolated from the cells and subjected to real-time RT-PCR analysis of Gimap5 gene expression in triplicate. Levels of Gimap5 transcription were presented as copies of Gimap5 transcripts per Actin transcript. Data shown are obtained from two independent experiments. (C) BM cells from wild-type (CD45.2⁺) or Gimap5-deficient mice (CD45.2⁺) were mixed 1:1 with wild-type competitor BM cells from B6 SJL mice (CD45.1⁺) and transplanted into lethally irradiated B6 SJL mice. 8 wk after BM transplantation, the recipient mice were analyzed for the relative contribution of donor engraftment. Data shown are representative of five recipients per donor group. (D) BM cells from CD45.2⁺ wild-type or Gimap5-deficient mice were transplanted into lethally irradiated CD45.1⁺ B6 SJL mice. 6 h later, the percentages of CD45.2⁺Lin⁻ donor hematopoietic progenitors in recipient BM were determined by FACS. Data shown are representative of four transplantations for each donor genotype. Error bars show ±SD.