Figure 2. Increased numbers of HSCs and markedly reduced numbers of primitive hematopoietic progenitors in Gimap5-deficient mice. (A) LSK and LK populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL-7R, c-Kit, and Sca-1 antibodies. The percentages indicate LSK and LK cells in a gated DAPI−lin−IL7R− population. (B) Bar graphs show the numbers of LSK and LK in BM of wild-type and Gimap5-deficient mice. (C) LT-SHC, ST-SHC, and MPP populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL7R, c-Kit, Sca-1, CD34, and CD135 antibodies. The percentages indicate LT-HSC (CD34−CD135−), ST-HSC (CD34+CD135−), and MPP (CD34+CD135+) in the gated LSK population. (D) Bar graphs show the numbers of LT-HSC, ST-HSC, and MPP in the BM of wild-type and Gimap5-deficient mice. (E) CMP, GMP, and MEP populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, Ter-119, and IL7R), c-Kit, Sca-1, CD34, and FcγR antibodies. The percentages indicate CMP, GMP, and MEP in the gated LK population. (F) Bar graphs show the numbers of CMP, GMP, and MEP cells in BM of wild-type and Gimap5-deficient mice. (G) CLP population in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL-7R, c-Kit, and Sca-1 antibodies. The percentages indicate CLP in the gated DAPI−lin−IL7R+ population. (H) Bar graphs show the numbers of CLP in BM of wild-type and Gimap5-deficient mice. Data shown are obtained from or representative of nine (A, B, G, and H) or seven (C–F) mice of each genotype. Error bars show ±SD. *, P < 0.001; **, P < 0.05.
Figure 2.

Increased numbers of HSCs and markedly reduced numbers of primitive hematopoietic progenitors in Gimap5-deficient mice. (A) LSK and LK populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL-7R, c-Kit, and Sca-1 antibodies. The percentages indicate LSK and LK cells in a gated DAPI−lin−IL7R− population. (B) Bar graphs show the numbers of LSK and LK in BM of wild-type and Gimap5-deficient mice. (C) LT-SHC, ST-SHC, and MPP populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL7R, c-Kit, Sca-1, CD34, and CD135 antibodies. The percentages indicate LT-HSC (CD34−CD135−), ST-HSC (CD34+CD135−), and MPP (CD34+CD135+) in the gated LSK population. (D) Bar graphs show the numbers of LT-HSC, ST-HSC, and MPP in the BM of wild-type and Gimap5-deficient mice. (E) CMP, GMP, and MEP populations in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, Ter-119, and IL7R), c-Kit, Sca-1, CD34, and FcγR antibodies. The percentages indicate CMP, GMP, and MEP in the gated LK population. (F) Bar graphs show the numbers of CMP, GMP, and MEP cells in BM of wild-type and Gimap5-deficient mice. (G) CLP population in Gimap5-deficient mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-lineage cocktail (Mac-1, Gr-1, B220, CD4, CD8, and Ter-119), IL-7R, c-Kit, and Sca-1 antibodies. The percentages indicate CLP in the gated DAPI−lin−IL7R+ population. (H) Bar graphs show the numbers of CLP in BM of wild-type and Gimap5-deficient mice. Data shown are obtained from or representative of nine (A, B, G, and H) or seven (C–F) mice of each genotype. Error bars show ±SD. *, P < 0.001; **, P < 0.05.

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