Impaired development of multiple hematopoietic lineages in Gimap5-deficient mice. (A) Bar graphs show the numbers of total BM cells in wild-type (+/+) and Gimap5-deficient (−/−) mice. (B) Bar graphs show the numbers of lymphoid (B220⁺and Thy1.2⁺), myeloid (Mac-1⁺Gr-1⁺), and erythroid (Ter119⁺) linage cells in BM of wild-type and Gimap5-deficient mice. (C) Body weight of 9–14-wk-old wild-type or Gimap5-deficient mice was measured. (D–I) Pro/pre–, immature, and mature B cells (D and E), immature and mature myeloid cells (F and G), and erythroid cells at different maturation stages (H and I) in the BM of Gimap5-deficient and wild-type mice. BM cells from wild-type or Gimap5-deficient mice were stained with anti-B220 and anti-IgM, anti–Mac-1 and anti–Gr-1, or anti-Ter119 and anti-CD71. The percentages (D, F, and H) and numbers (E, G, and I) of the indicated cells in total living BM cells are shown. Data shown are obtained from or representative of 9 (A, B, and D–I) or 14 (C) mice of each genotype. Error bars show ±SD. *, P < 0.001; **, P < 0.05.