Figure 5.
Preferential IFN-γ secretion of the Ly49L+ NK cell subpopulation at day 6 p.i. in BALB.K mice. BALB.By, BALB/c, and BALB.K mice were infected with 2,500 PFU/mouse of MCMV i.p. At 36 h and 6 d p.i., the spleens were collected, negatively enriched for NK cells, and activated with plate-bound α-NKp46 and α-NKG2D antibody followed by CD3, DX5, YE1/48, 12A8, and IFN-γ staining. Percentages of total IFN-γ+ NK cells gated on CD3−DX5+ are shown at 36 h (A) and 6 d (B) p.i. (C) Representative contour plots of 12A8−YE1/48INTIFN-γ+ NK cells. Data represent one of two experiments performed (mean ± SD) with three to four mice per group.