Figure 4. H-2 and m04/gp34-specific proliferation of Ly49L+ NK cells in BALB mice during MCMV infection. (A) 2B4 reporter cells expressing Ly49 receptors from the BALB repertoire were stained with a panel of Ly49-specific antibodies as well as with anti-M2 (DAP12) antibody. MFI is shown. (B) Freshly extracted splenocytes from BALB congenic mice were stained for NK cells and different Ly49 receptors. YE1/48 and 12A8 antibody staining is shown. YE1/48 stains a double population of NK cells with either high or intermediate intensity. The intermediate population is not stained by the anti-Ly49A–specific clone (12A8) and represents the fraction of NK cells expressing the activating Ly49L receptor. BALB.By, BALB/c, or BALB.K mice were infected with WT or Δm04 MCMV. Their spleens were collected at different time points p.i. (C and D) The number of NK cells (C) and the percentages of Ly49A-, Ly49C-, Ly49G-, or Ly49L-positive NK cell fractions (D) were determined. Asterisks denote p-values <0.05. (E) Representative dot plots of Ly49A and Ly49L expression on NK cells from BALB.By, BALB/c, or BALB.K mice infected with WT MCMV at day 4 and 6 p.i. Data are from three experiments (mean ± SD) with three to four mice per group. n/a, not available.
Figure 4.

H-2 and m04/gp34-specific proliferation of Ly49L+ NK cells in BALB mice during MCMV infection. (A) 2B4 reporter cells expressing Ly49 receptors from the BALB repertoire were stained with a panel of Ly49-specific antibodies as well as with anti-M2 (DAP12) antibody. MFI is shown. (B) Freshly extracted splenocytes from BALB congenic mice were stained for NK cells and different Ly49 receptors. YE1/48 and 12A8 antibody staining is shown. YE1/48 stains a double population of NK cells with either high or intermediate intensity. The intermediate population is not stained by the anti-Ly49A–specific clone (12A8) and represents the fraction of NK cells expressing the activating Ly49L receptor. BALB.By, BALB/c, or BALB.K mice were infected with WT or Δm04 MCMV. Their spleens were collected at different time points p.i. (C and D) The number of NK cells (C) and the percentages of Ly49A-, Ly49C-, Ly49G-, or Ly49L-positive NK cell fractions (D) were determined. Asterisks denote p-values <0.05. (E) Representative dot plots of Ly49A and Ly49L expression on NK cells from BALB.By, BALB/c, or BALB.K mice infected with WT MCMV at day 4 and 6 p.i. Data are from three experiments (mean ± SD) with three to four mice per group. n/a, not available.

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