CD11b⁺Gr1⁺ cells accumulate in the blood and BM of Vav-Atg7^−/− and express higher levels of the proliferation marker Ki67. (A) Peripheral blood cells from 7-wk-old WT and asymptomatic Vav-Atg7^−/− mice were stained with antibodies specific for CD11b and Gr1. (B) Representative CD11b and Gr1 staining of 9-wk-old WT (Vav-iCre⁺; Atg7^Flox/WT or Vav-iCre⁻; Atg7^Flox/Flox) and asymptomatic Vav-Atg7^−/− (Vav-iCre⁺; Atg7^Flox/Flox) BM. Numbers in the dot plots indicate the percentage of cells within each gate. Dot plots are representative of at least three independent experiments. (C) Ki67 staining of CD11b⁺Gr1⁺ BM cells (gated as shown in B) from 9-wk-old WT (n = 5) and Vav-Atg7^−/− (n = 4) mice. *, P < 0.05 from Mann-Whitney test on the fluorescence geometric mean of Ki67.