LSK cells from Vav-Atg7^−/− mice accumulate mitochondria, mitochondrial superoxide, and DNA damage and display increased apoptosis and proliferation. (A–F) LSK cells from WT (Vav-iCre⁺; Atg7^Flox/WT or Vav-iCre⁻; Atg7^Flox/Flox) and Vav-Atg7^−/− (Vav-iCre⁺; Atg7^Flox/Flox) mice were stained with MitoTracker green (MTG; A), MitoTracker red (MTR; B), MitoSOX (C), 53BP1 (D), active caspase 3 (E), and Ki67 (F). A–C represent BM cells from 9-wk-old WT (n = 4) and Vav-Atg7^−/− (n = 4) mice. D and F represent BM cells from 7-wk-old WT (n = 2) and Vav-Atg7^−/− (n = 2) mice. In E, BM from 6-wk-old WT (n = 5) and Vav-Atg7^−/− (n = 3) mice was analyzed. *, P < 0.05 from Mann-Whitney tests on the fluorescence geometric mean of the indicated dye/antibody. Autofluorescence levels were confirmed to be similar in WT and Vav-Atg7^−/− cells using unstained, single stained, and fluorescence minus one controls on both cell types.