Hematopoietic progenitors of multiple lineages are reduced in the BM of Vav-Atg7^−/− mice. BM cells from WT (Vav-iCre⁺; Atg7^Flox/WT or Vav-iCre⁻; Atg7^Flox/Flox) and Vav-Atg7^−/− (Vav-iCre⁺; Atg7^Flox/Flox) mice were stained with the indicated antibodies and analyzed by flow cytometry. (A) CLPs were identified as Sca-1^Lowc-Kit^Low cells within Lin⁻Flt3^HighIL7Ra^High cells. (B) Absolute BM CLP counts were determined by gating as shown in A. (C) CCR9⁺ LMPPs were identified as Flt3^HighCCR9⁺ cells within LSK cells. (D) Absolute CCR9⁺ LMPPs counts were determined by gating as shown in C. (E) NKPs were identified as NK1.1⁻DX5⁻ cells within Lin⁻CD3⁻CD122⁺ cells, and immature NK cells were identified as NK1.1⁺DX5⁺ cells within Lin⁻CD3⁻CD122⁺ cells. (F) Absolute BM NKP and immature NK cell counts were determined by gating as shown in E. Representative plots of WT (left) and Vav-Atg7^−/− (right) BM are shown in A, C, and E. (B, D, and F) Horizontal bars indicate the mean (**, P < 0.01; ns, nonsignificant). (G) The LK myeloid progenitor compartment was immunophenotyped as described in Pronk et al. (2007). The gating strategies applied are shown for WT (top) and Vav-Atg7^−/− BM (bottom). CFU-E, CFU erythroid; GMP, granulocyte-macrophage progenitor; MkP, megakaryocyte progenitor. (H) Absolute cell counts of BM myeloid progenitors were determined by gating as shown in G. The numbers in dot plots indicate the percentage within the corresponding parent population. Error bars indicate SEM (**, P < 0.01; ns, nonsignificant from Mann-Whitney tests).