CCL21 depositions are concentrated in specific regions of LECs. Whole-mount confocal images of the skin of the footpad (a) or ear (b) stained for LYVE-1 and CCL21 showed a punctate expression of CCL21 on initial lymphatics (blue dots). (c) This pattern was even more obvious after isosurface rendering. (d and e) Triple staining for VE-cadherin, LYVE-1, and CCL21 shows alternate arrangement of VE-cadherin at button junctions and LYVE-1 at loose flaps. CCL21 puncta are located in the junction-free areas of the LECs. (f–h) Compared with the steady-state, inflammation, either through CHS (g) or CFA (h), did not affect the pattern of CCL21 puncta or increase the CCL21 signal after 24 h.