Figure 1. Optical sectioning of the intact footpad skin from a live CD11c-EYFP mouse. (a–d) Images representing extended focus views of subsequent layers. (a) The upper layer of the epidermis, at 0–20 µm in depth, consists of cornified keratinocytes stained topically with SNARF. (b) The epidermis, at ∼20–40 µm, contains CD11c-EYFP+ star-shaped LCs. (c) The upper dermis, at ∼40–65 µm, contains collagen fibers, which generate a second harmonic signal, and blood vessels traced with QD655. (d) The deeper dermis, at ∼65–120 µm, contains CD11c-EYFP+ DDCs and lymphatics stained with anti-LYVE1 conjugated to Texas Red. (e–g) Three-dimensional reconstructions of all the above layers (e), epidermal LCs (f), and dermal lymphatics, DDCs and blood vessels shown from below (g). Images are representative of at least 15 paws of CD11c-EYFP+ mice examined.
Figure 1.

Optical sectioning of the intact footpad skin from a live CD11c-EYFP mouse. (a–d) Images representing extended focus views of subsequent layers. (a) The upper layer of the epidermis, at 0–20 µm in depth, consists of cornified keratinocytes stained topically with SNARF. (b) The epidermis, at ∼20–40 µm, contains CD11c-EYFP+ star-shaped LCs. (c) The upper dermis, at ∼40–65 µm, contains collagen fibers, which generate a second harmonic signal, and blood vessels traced with QD655. (d) The deeper dermis, at ∼65–120 µm, contains CD11c-EYFP+ DDCs and lymphatics stained with anti-LYVE1 conjugated to Texas Red. (e–g) Three-dimensional reconstructions of all the above layers (e), epidermal LCs (f), and dermal lymphatics, DDCs and blood vessels shown from below (g). Images are representative of at least 15 paws of CD11c-EYFP+ mice examined.

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