Immunofluorescence staining analysis of TDP-43 distribution in the neurons of the mouse brains. (A) The brain sections of the WT and Tg mice were costained with anti–TDP-43 (red), anti-NeuN (green), and DAPI (blue). TDP-43⁺ NCIs are indicated by arrows. One neuron each in the Tg^+/+ and WT samples (boxed) was magnified in the lower right corners (n = 5 mice/group). Bars, 20 µm. (B) Representative immunostaining pattern of a 6-mo-old Tg^+/+ mouse brain section exhibiting neuronal cells with TDP-43 (green)-containing NCIs that are also positive for the anti-ubiquitin (Ub) staining (red, arrows). High magnification photos of one of the cells with TDP-43⁺, Ub⁺ NCIs are shown in the lower right corners . Bars, 20 µm.