Alterations of the levels of learning/memory-associated proteins in CaMKII-TDP-43 Tg mice. (A) Western blot analysis of different proteins in extracts prepared from isolated cerebral cortex and hippocampus of 2-mo-old WT and Tg mice (n = 2 mice/group). Results are representative of three independent experiments. Quantitative analysis of the Western blot data are shown in the lower portion of A. (B) Immunostaining analysis of GAD67 expression and measurement of GABA release. The primary hippocampal neuron cultures of the WT (left) and TDP-43 Tg (Tg, right) mice were double-stained with anti- GAD67 (green) and anti–TDP-43 (red). Bars, 50 µm. The statistical comparison of the relative GAD67 intensities of GAD67⁺ cells of the WT and Tg mice is shown in the left histogram below the confocal images. The data represent the mean ± SEM of three independent experiments (n = 20 mice/group). Shown in the lower right histogram is the statistical comparison of the GABA levels of the WT and Tg mice (n = 5; P < 0.05).