Naive T cell infiltration and activation within tumors extends to different tumor types and T cell avidities. C57BL/6 mice bearing established s.c. or i.p. LLC-OVA tumors (A) or AAD⁺ mice bearing established s.c., lung, or i.p. B16-AAD tumors (B) received Thy1-mismatched OT-I or FH T cells, respectively. 24 h later, tumors, LN, and spleen were harvested and stained for flow cytometry. Plots are gated on Thy1.1⁺ CD8⁺ OT-I cells (A) or Thy1.2⁺ CD8⁺ FH cells (B). Tumor location is indicated to the right of dot plots. Numbers indicate the percentage of OT-I or FH cells that are single positive for CD69 or CD25, or positive for both markers. Axes on plots are displayed in Logicle scale. Results represent at least two independent experiments with three or more mice each for each tumor type and location.