EAE is exacerbated in mice lacking MHCII expression by pDCs. (A) LN cells from WT:WT and pIII+IV^−/−:WT chimeras and control WT and pIII+IV^−/− mice were analyzed by flow cytometry for the presence of CD4⁺ T cells. Percentages of CD4⁺ T cells are indicated. Results are representative of 10 experiments, each with at least three mice per group. (B) MHCII (I-A^b) expression by LN cDCs, B cells, and pDCs was analyzed by flow cytometry for pIII+IV^−/−:WT, H2-Aa^−/−:WT, and WT:WT chimeras. Results are representative of five experiments, each with at least three mice per group. (C) EAE was induced by immunization with MOG_35–55+CFA in pIII+IV^−/−:WT (n = 8), µMT:WT (n = 6), and WT:WT (n = 6) chimeras. Results are representative of three experiments. The means and SEM are shown. ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. (D) LN cells from WT:WT, µMT:WT, and µMTxpIII+IV^−/−:WT chimeras were analyzed by flow cytometry for the expression of CD11c and B220. The percentages of B220⁺ B cells, CD11c^hi cDCs, and CD11c^intB220⁺ pDCs are indicated. Results are representative of three experiments, each with at least three mice per group. (E) MHCII (I-A^b) expression by LN cDCs and pDCs was analyzed by flow cytometry for µMT:WT, µMTxpIII+IV^−/−:WT, and WT:WT chimeras. Results are representative of three experiments, each with at least three mice per group. (F) EAE was induced by immunization with MOG_35–55+CFA in µMTxpIII+IV^−/−:WT and µMT:WT chimeras. Results are representative of two experiments. The means and SEM derived from six mice per group are shown. **, P < 0.01; *, P < 0.05. (G) pDCs from WT, pIII+IV^−/−, and H2-Aa^−/− mice were incubated with MOG_35–55 and transferred into WT recipients. EAE was induced 1 d after the transfer by immunization with MOG_35–55. Results are representative of two independent experiments. The means and SEM derived from five mice per group are shown. **, P < 0.01; *, P < 0.05.