Colons of Bcl11b^F/F/CD4-Cre mice accumulate proinflammatory cytokine–producing CD4⁺ T cells, neutrophils, dendritic cells, and macrophages. (A and B) Intracellular cytokine production by CD4⁺ T cells isolated from the colon LP (A) and epithelium (IEL; B) of Bcl11b^F/F/CD4-Cre and WT mice. (C) Intracellular cytokine production by colon IEL CD4⁺ CD8⁺ T cells. (A–C) Cells were isolated and activated as described in Materials and methods, followed by staining for surface CD4 and CD8 and intracellularly for the indicated cytokines, and analyzed by flow cytometry. Frequencies of cytokine-producing cells are indicated within the gated CD4⁺ T or CD4⁺ CD8⁺ T cell population. (D and E) Frequencies of neutrophils, dendritic cells, and macrophages in the colon LP (D) and epithelium compartment (IEL; E) are shown. Cells were isolated as described in Materials and methods, stained for CD4, CD8, Gr1, CD11c, and F4/80, and analyzed by flow cytometry. The numbers in the dot plots and histograms indicate the percentage of Gr1^hi, CD11c⁺, and F4/80⁺ populations, respectively, within the gated CD4⁻CD8⁻ population. The data are representative of four to five pairs of mice.