Intestinal architecture, epithelial proliferation, and gene expression in unchallenged ADAM17^ex/ex mice. (A) H&E staining of paraffin-embedded colon tissue from unchallenged ADAM17^ex/ex mice. Bar, 100 µM. Representative microscopic pictures of eight ADAM17^ex/ex mice are shown. (B) ADAM17^ex/ex (n = 3) and WT (n = 3) mice were injected intraperitoneally with BrdU and stained for BrdU in the distal colon. The arrowheads indicate BrdU-positive nuclei. Bars, 100 µm. The experiment was performed three times with three animals per group. (C) Pathway analysis of microarray data from colon tissue of WT (n = 3) and ADAM17^ex/ex (n = 3) mice. Regulated genes, which are significantly enriched or depleted during biological processes, are displayed. The bar represents the −log(p) of the probability of the enrichment or depletion occurring by chance. Processes that are associated with down-regulated transcripts are shown on the left, and those associated with up-regulated transcripts are shown on the right. The numbers in the bars represent the number of observed regulated transcripts in the category.