Differences in CD8⁺ T cells from Ly49H⁻ and Ly49H⁺ mice. Ly49H⁻ and Ly49H⁺ mice were infected with 5 × 10³ PFU MCMV and splenocytes harvested at day 6 and 10 pi. (A) Expression of CD69 was assessed on live TCR⁺CD8⁺IE1-tetramer⁺ cells. The frequency of CD69⁺ CD8⁺IE1-tetramer⁺ cells is plotted. Frequencies are shown as arithmetic means with standard errors. The data are representative of two independent experiments each with three mice per group. (B) The frequencies of IFN-γ⁺TNF-α⁺ CD8⁺IE1⁺ cells are plotted. The data are representative of two independent experiments each with three mice per group. Frequencies are shown as arithmetic means with standard errors. Experiments were performed in BALB/c (Ly49H⁻) and BALB.B6-CT8 (Ly49H⁺) mice.