Figure 1.
Figure 1. NK cells affect antiviral CTL function. Ly49H− and Ly49H+ mice were infected with MCMV (5 × 103 PFU K181-Perth). (A) Virus-specific CTL activity was assessed in vivo at days 2, 4, 6, 8, and 10 pi by measuring the elimination of adoptively transferred CFSE low targets pulsed with the IE1 viral peptide. Unpulsed (CFSE high) targets are used for comparison. The histograms represent the number of CFSE high (unpulsed) and CFSE low (IE1 pulsed) targets in the spleens of relevant mice. The data are representative of at least two experiments each using three mice per time point. (bottom) CTL data are shown as percentage of IE1-specific cytotoxicity determined as described in the Materials and methods section. Arithmetic means and standard errors were plotted using the results from two independent experiments each using three mice per time-point. (B) Ly49H−, Ly49H+ isotype-matched control Ig-treated mice, and Ly49H+ anti-NK1.1–treated mice were infected with MCMV (5 × 103 PFU). Virus-specific CTL activity was measured in vivo at days 6 and 10 pi. Data are representative of at least five independent experiments each using three mice per time point. (right) CTL data are shown as percentage of IE1-specific cytotoxicity. Arithmetic means and standard errors were plotted using the results from two independent experiments each using three mice per time point. (C) Ly49H+ mice were infected with wild-type MCMV (MCMV-m157wt) or a mutant MCMV virus with a nonfunctional m157 glycoprotein (MCMV-m157mut). CTL data are plotted as percentage of IE1-specific cytotoxicity. CTL data from Ly49H− mice infected with wild-type MCMV are shown for comparison. Arithmetic means and standard errors are plotted. The data are representative of at least three independent experiments each with at least three mice per group (D) Virus-specific CTL activity was measured at days 18, 25, 32, and 40 after infection in ▪ (Ly49H−) and □ (Ly49H+) mice. Data are representative of two independent experiments each using 3 mice per group. Arithmetic means and standard errors are plotted. The differences were significant at all times. P < 0.0005. Experiments described in A–C were performed in BALB/c (Ly49H−) and BALB.B6-Cmv1r (Ly49H+) mice. Experiments described in D were performed in BALB/c (Ly49H−) and BALB.B6-CT8 (Ly49H+) mice.

NK cells affect antiviral CTL function. Ly49H− and Ly49H+ mice were infected with MCMV (5 × 103 PFU K181-Perth). (A) Virus-specific CTL activity was assessed in vivo at days 2, 4, 6, 8, and 10 pi by measuring the elimination of adoptively transferred CFSE low targets pulsed with the IE1 viral peptide. Unpulsed (CFSE high) targets are used for comparison. The histograms represent the number of CFSE high (unpulsed) and CFSE low (IE1 pulsed) targets in the spleens of relevant mice. The data are representative of at least two experiments each using three mice per time point. (bottom) CTL data are shown as percentage of IE1-specific cytotoxicity determined as described in the Materials and methods section. Arithmetic means and standard errors were plotted using the results from two independent experiments each using three mice per time-point. (B) Ly49H−, Ly49H+ isotype-matched control Ig-treated mice, and Ly49H+ anti-NK1.1–treated mice were infected with MCMV (5 × 103 PFU). Virus-specific CTL activity was measured in vivo at days 6 and 10 pi. Data are representative of at least five independent experiments each using three mice per time point. (right) CTL data are shown as percentage of IE1-specific cytotoxicity. Arithmetic means and standard errors were plotted using the results from two independent experiments each using three mice per time point. (C) Ly49H+ mice were infected with wild-type MCMV (MCMV-m157wt) or a mutant MCMV virus with a nonfunctional m157 glycoprotein (MCMV-m157mut). CTL data are plotted as percentage of IE1-specific cytotoxicity. CTL data from Ly49H− mice infected with wild-type MCMV are shown for comparison. Arithmetic means and standard errors are plotted. The data are representative of at least three independent experiments each with at least three mice per group (D) Virus-specific CTL activity was measured at days 18, 25, 32, and 40 after infection in ▪ (Ly49H−) and □ (Ly49H+) mice. Data are representative of two independent experiments each using 3 mice per group. Arithmetic means and standard errors are plotted. The differences were significant at all times. P < 0.0005. Experiments described in A–C were performed in BALB/c (Ly49H−) and BALB.B6-Cmv1r (Ly49H+) mice. Experiments described in D were performed in BALB/c (Ly49H−) and BALB.B6-CT8 (Ly49H+) mice.

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