Proliferation and infiltration of ITK-SYK–expressing T cells upon transplantation. (A) Splenic cells from diseased ITK-SYKCD4-Cre animals older than 12 wk of age were intravenously injected into nude recipient mice for transplantation. The frequencies of ITK-SYK–expressing eGFP+ peripheral blood cells in the recipients were monitored over time. Recipients that succumbed to the disease are indicated (†). Representative examples are shown. 9 out of 10 donor mice transplanted the disease. Five independent transplantations were performed. (B) Spleens from a control and a representative recipient mouse from A are shown (in centimeters). (C) Recipients were sacrificed upon signs of disease, and bone marrow (BM) and spleen (SPL) suspensions were stained against TCR-β. Representative frequencies of eGFP fluorescent T cells in spleen and bone marrow of 10 analyzed diseased recipients are indicated. (D) Tissue sections from spleen (SPL), liver (LIV), and lung (LNG) were analyzed after H&E staining or immunohistochemistry with anti-CD3 or anti-Ki-67 antibodies. Bars, 1 mm. Representative examples of four analyzed diseased recipients are shown.
