A Vα14i transgene rescues the development of Vα14i NKT cells in CD8 Tg/Tg mice. (A) Flow cytometric analysis of the indicated electronically gated populations from the spleens of littermate controls, Vα14i Tg, CD8 Tg/Tg, and Vα14i Tg + CD8 Tg/Tg double transgenic mice depicting the staining with αGalCer-CD1d tetramers and anti–TCR-β, as well as the staining of cells within the Vα14i NKT cell gate for CD44 and NK1.1. Data are representative of two separate experiments involving five Vα14i Tg + CD8 Tg/Tg double transgenic mice and similar numbers of controls. (B) Flow cytometric analysis of intracellular cytokine expression by liver CD44^high Vα14i NKT cells from Vα14i Tg + CD8 Tg/Tg double transgenic, Vα14i Tg, and control WT mice analyzed ex vivo 90 min after injection of αGalCer. Data are representative of three Vα14i Tg + CD8 Tg/Tg double transgenic Vα14i mice.