Reduced levels of iNKT cells are sufficient to see increased autoreactive B cell activation to injected apoptotic cells, and this can be rescued by transferring iNKT cells. (A) FACS analysis of the splenic iNKT cell population (TCRβ⁺ and αGalCer loaded CD1d-dimer⁺) in WT, Jα18^+/− and Jα18^−/− mice. (B) IgG anti-DNA response and (C) analysis of splenic and lymph node GCs after four injections of apoptotic cells in mice described in (A). (D) Schematic illustration of the iNKT cell transfer to a Jα18^−/− recipient followed by four injections of apoptotic cells. (E) IgG3 anti-DNA response in mice described in (D) measured by ELISA. (F) Quantification of splenic GC B cells with FACS day 26. (G) Representative histology of splenic GCs (GL7⁺, indicated by arrows) day 26. Bars, 300 µm. Results are shown as individual mice and mean in A (n = 3), C (n = 1–7), and F (n = 7) and mean ± SEM in B (n = 7) and E (n = 7). *, P < 0.05; **, P < 0.01. Data are representative of two independent experiments (A–C) or pooled from two independent experiments (D–G).