CD103⁺MHCII^hi DCs prime naive myelin-specific T cells and induce Th effector cell differentiation. (A) DC subsets sorted from cutaneous lymph nodes of WT mice 20 h after immunization with MOG/CFA were cultured with purified, CFSE-stained CD45.1⁺CD4⁺ 2D2 T cells in the absence or presence of MOG peptide. Anti–I-A^b antibodies were added to some wells. Plots are gated on CD45.1⁺ T cells. Percentages are shown. Data are representative of four independent experiments. (B) Sorted naive 2D2 T cells were cultured with sorted lymph node DC subsets or unfractionated lymph node DCs in the presence of MOG peptide for 4 d. Cells were restimulated with anti-CD3/-CD28 for 48 h for detection of IFN-γ or IL-17 in supernatants by ELISA (error bars represent SEM). (C) Cells were prepared as described in B but harvested after 96 h of primary culture for real-time RT-PCR analysis. Data shown in B and C are representative of three separate experiments.