Radiosensitive langerin⁺CD103⁺ DCs promote encephalitogenic Th1/Th17 responses and induction of EAE. (A, top) Langerin-DTR→WT chimeric mice were treated with vehicle (left) or DT (right) for 2 d before analysis of cutaneous lymph node cells by flow cytometry. Dot plots are gated on CD11c⁺MHCII⁺ cells. (bottom) Gating on langerin⁺MHCII^hi lymph node cells from MOG-immunized chimeric mice. Percentages are shown. (B) Clinical course of EAE in chimeric mice injected with DT or vehicle alone. Data shown are combined from two separate experiments with five or more mice per group (*, P < 0.05; **, P < 0.002; N.S., not statistically significant; error bars represent SEM). (C) ELISPOT analysis of draining lymph nodes 6 d after immunization of DT-treated chimeric or WT mice and vehicle-treated chimeric mice (*, P < 0.05; **, P < 0.01; error bars represent SEM). Data are representative of two independent experiments with five mice per group.