Langerin⁺CD103⁺MHCII^hi DCs require GM-CSF signaling to accumulate in the peripheral lymph nodes of MOG-immunized mice. (A) Percentages of CD11c⁺ subsets in draining lymph nodes of WT, GM-CSF^−/−, or β_c^−/− mice on day 7 after immunization with MOG/CFA. Histograms are based on the gates illustrated in the dot plots (left). (far right) Dot plots are gated on all CD11c⁺MHCII⁺ cells. (B and C) Absolute number of total CD11c⁺MHCII⁺ cells (B) and DC subsets (C) in draining lymph nodes on day 7 after immunization with MOG/CFA (*, P < 0.05; n.s., not statistically significant; error bars represent SD). (D) Cell-surface profiles of CD103⁺ and CD103⁻MHCII^hi lymph node DCs from MOG-immunized WT mice. Shaded histograms indicate background staining. (E) CD45.2 expression on langerin⁺ DC subsets in cutaneous lymph nodes of CD45.2⁺→CD45.1⁺ bone marrow chimeras. Percentages are shown in D and E. Data are representative of three independent experiments with three or more mice per group.