Dysgammaglobulinemia in Batf^ΔZ/ΔZ mice. (A) ELISA was performed to measure the indicated Ig in sera of Batf^+/+ and Batf^ΔZ/ΔZ mice. Shown are mean results from four mice per group (n = 4) assayed in duplicate. Error bars indicate SE. *, P < 0.05. (B) Sera from Batf^+/+ and Batf^ΔZ/ΔZ mice, immunized with sRBC or mock injected with PBS, were used in ELISA to detect sRBC-specific IgM or IgG. Mean results from one (n = 3) of two experiments are shown. Error bars indicate SE. *, P < 0.05. (C) Spleen sections from mice in B (n = 3 for each genotype) were incubated with primary anti-IgG1 and anti-IgG2c Abs. Complexes were detected using biotinylated secondary Abs and Vectastain ABC reagent. Shown are representative images, counterstained with H (no E) and photographed at 40×. f, follicle. Bars, 50 µm. (D) Spleen sections from mice in B (n = 3 for each genotype) were stained with H + E (left) and photographed at 4× (bars, 250 µm) or 20× (insets; bars, 50 µm). GCs (arrows) were detected on additional sections (right) using biotinylated PNA and Vectastain ABC reagent, counterstained with H (no E; 20×; bars, 50 µm) or using biotinylated PNA, anti–mouse B220, DAPI, and fluorescently labeled secondary mAbs (60×; bars, 50 µm).