CD11c, not basophil, depletion disrupts Th2 induction in schistosome egg-challenged mice. CD11c.DOG x 4get mice were treated daily with PBS (squares) or DTx (triangles) from day −2 to 6. On day −1, 1, and 3 mice were also treated with IgG (black symbols) or Mar-1 (gray symbols). On day 0, mice were challenged with S. mansoni eggs or PBS. pLN CD11c^HiMHCII⁺ cell depletion was assessed on day 7 (A and B), and IL-4-eGFP⁺B220⁻CD4⁻CCR3⁻CD117⁻ basophil depletion in the blood on day 4 (C and D), after egg injection. On day 7, pLN cells from naive or egg-injected, PBS (white or black bars) or DTx (gray bars), IgG or Mar-1–treated mice were cultured for 72 h with SEA or medium alone. The supernatants were collected, and SEA-specific cytokine production (medium alone values subtracted) was assessed by ELISA (E and F). pLN cells were also assessed on day 7 for IL-4-eGFP expression (G and H). One of three experiments. Error bars are mean ± SEM of four to seven mice/group.