Phenotypic changes associated with resetting. (a) 10 d after transfer of CFSE-labeled splenocytes, the frequencies of CIN⁺ and CIN⁻ NK cells were determined among gated donor NK cells that remained undivided after transfer by staining with Ly49C, Ly49I, and NKG2A antibodies. (b) IFN-γ response of KLRG1^high and KLRG1^low WT NK cells to stimulation with 5 µg/ml plate-bound NKG2D antibody. (c) Percentages of KLRG1^high cells among undivided donor NK cells at day 10 after transfer. (d) IFN-γ response of CD27⁻ CD11b⁻, CD27⁺ CD11b⁻, CD27⁺ CD11b⁺, and CD27⁻ CD11b⁺ NK cell subsets from WT mice upon stimulation with 5 µg/ml anti-NKG2D. (e) Distribution of undivided donor NK cells among the CD27/CD11b-defined subsets at day 10 after transfer. Asterisks indicate a statistically significant difference as calculated by two-tailed paired (b) or unpaired (c) Student’s t test (*, P < 0.05; ***, P < 0.0005). Experiments were performed two to four times with n = 3–6 mice, except one repeat of panel d where n = 2 mice. Data represent means ± SEM.