Figure 2.
Figure 2. Hyporesponsive NK cells reset their functional potential upwards when exposed to MHC I–expressing cells. (a–c) Responses of β2m−/− NK cells after transfer to MHC I–expressing NK cell–deficient (NKD) or β2m-deficient hosts. Stimulations and assays were performed as in Fig. 1. The percentage of IFN-γ–producing NK cells among gated donor NK cells (a and b) or gated donor CIN+ and CIN− NK cells (c) was determined by flow cytometry. Recipients were either left untreated (a) or treated with Poly I:C (b and c) 36 h before in vitro stimulation to augment the responses. Experiments were repeated four times with n = 3–6 mice each for transfer groups. For relevant comparisons, statistical significance was determined with a two-tailed unpaired (a and b) or paired (c) Student’s t test (*, P < 0.05; **, P < 0.005; ***, P < 0.0005). Data represent means ± SEM. ctrls, controls.

Hyporesponsive NK cells reset their functional potential upwards when exposed to MHC I–expressing cells. (a–c) Responses of β2m−/− NK cells after transfer to MHC I–expressing NK cell–deficient (NKD) or β2m-deficient hosts. Stimulations and assays were performed as in Fig. 1. The percentage of IFN-γ–producing NK cells among gated donor NK cells (a and b) or gated donor CIN+ and CIN− NK cells (c) was determined by flow cytometry. Recipients were either left untreated (a) or treated with Poly I:C (b and c) 36 h before in vitro stimulation to augment the responses. Experiments were repeated four times with n = 3–6 mice each for transfer groups. For relevant comparisons, statistical significance was determined with a two-tailed unpaired (a and b) or paired (c) Student’s t test (*, P < 0.05; **, P < 0.005; ***, P < 0.0005). Data represent means ± SEM. ctrls, controls.

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