Figure 2.
P2X7 deficiency abrogates the sensitization capacity of BMDCs and is restored by alum pretreatment. (A and B) WT (A) or P2X7−/− (B) mice were sensitized by i.c. injection of 3 × 105 unmodified or TNP-modified WT or P2X7−/− BMDCs into the shaved abdominal skin. Ear challenge was performed as in Fig. 1. (C and D) The experiment was performed as described in A and B, but the indicated BMDCs were prestimulated or not with alum for 2 h before adoptive transfer. WT (C) or P2X7−/− mice (D) were sensitized by i.c. injection of 3 × 105 BMDCs. (A–D) The data represent the mean increase in ear thickness of groups of five mice ± SD. One representative of three independent experiments is shown (***, P < 0.001).
