NO inhibition or stimulation does not impair the ability of tumors to seed the lung and liver. (A and B) WT mice were inoculated intravenously (A) or intrasplenically (B) with Renca tumor. One-half of the mice were treated with L-NAME for the duration of the experiment. (A–C) WT mice were inoculated intravenously with Renca tumor and treated with either JS-43-126 or JS-K on days 1, 3, 6, 8, 10, and 13 after tumor injection. On day 14, mice were euthanized and the lungs (A and C) and livers (B) were collected and fixed in Bouin’s solution. The number of tumor nodules in each organ was determined by counting under a dissecting microscope. Each data point represents the results from an individual mouse and each treatment group contained at least eight mice. The horizontal bars indicate the means for each treatment group. The data are derived from one experiment. The results in A and B are representative of two separate experiments. NS = not significant.